Quality control in hematology

All Chemicals & Bioassays Resources…

BLAST (Basic Local Alignment Search Tool)

BLAST (Basic Local Alignment Search Tool)

Conserved Domain Search Service (CD Search)

Vector Alignment Search Tool (VAST)

Conserved Domain Search Service (CD Search)

Structure (Molecular Modeling Database)

Vector Alignment Search Tool (VAST)

All Domains & Structures Resources…

Database of Genotypes and Phenotypes (dbGaP)

Gene Expression Omnibus (GEO) Database

Gene Expression Omnibus (GEO) Datasets

Gene Expression Omnibus (GEO) Profiles

Online Mendelian Inheritance in Man (OMIM)

All Genes & Expression Resources…

Database of Genotypes and Phenotypes (dbGaP)

Online Mendelian Inheritance in Man (OMIM)

All Genetics & Medicine Resources…

Database of Genomic Structural Variation (dbVar)

BLAST (Basic Local Alignment Search Tool)

Conserved Domain Search Service (CD Search)

BLAST (Basic Local Alignment Search Tool)

Conserved Domain Search Service (CD Search)

BLAST (Basic Local Alignment Search Tool)

Conserved Domain Search Service (CD Search)

All Training & Tutorials Resources…

Database of Genomic Structural Variation (dbVar)

Database of Genotypes and Phenotypes (dbGaP)

Database of Single Nucleotide Polymorphisms (dbSNP)

Generate a file for use with external citation management software.

A quality control (QC) protocol for hematology, as for other sections of the laboratory, should encompass both internal and external QC programs. The extent to which a hematology laboratory should be involved depends upon various factors, including availability of facilities, financial resources, range of tests, workload, the number of staff and their levels of training, and the overall organization of the laboratory. To ensure quality patient care, the intralaboratory QC program must include at least the minimal measures of monitoring and control at each step from collection of blood specimens, through the actual processing and analysis, to reporting of the results. The protocol should be written concisely and in simple language; the procedure manual should offer all of the pertinent information along with references; all concerned personnel should be well trained and competent; and adequate facilities and time should be available for the purpose of QC. Continuing education is also an integral part of an effective QC program. Three very important aspects of QC in hematology are calibration of automated instruments, monitoring of accuracy and precision of instruments and procedures, and verifying the reliability of test results. In the absence of a true primary reference/standard for calibration of instruments for the CBC, the most commonly performed hematologic test, the use of commercial calibrators is acceptable. A combination of commercial controls (three levels) and retained or fresh patient blood specimens is recommended for monitoring of accuracy and precision on a long- and short-term basis. Patient red-cell indices moving average data allow continuous monitoring of instrument performance and should be used as an adjunct to other QC approaches to detecting instrument calibration drift. Correlation of results of related parameters and careful review of blood films remain the two most important and widely used approaches to ensure reliability of results obtained from automated hematology instruments. Participation in an external QC program offers the most practical means of monitoring overall work performance in comparison with instrument, method, and/or reagent-based peer group data. A laboratory may choose to participate in one or more national and/or regional QC programs, depending upon the range of tests it performs and the requirements of accreditation and regulatory agencies. Most of the accreditation agencies require participation in programs covering at least all of the routinely or frequently performed tests and, if available, also in those for infrequently performed tests.(ABSTRACT TRUNCATED AT 400 WORDS).

National Center for Biotechnology Information,U.S. National Library of Medicine8600 Rockville Pike,BethesdaMD,20894USA

Fresh Food Market Convenience Store Quality Fuel

I accidentally locked my keys and cell phone in the car at your store. One of your Team Members immediately offered his cell phone to call for assistance, and was able to get the door unlocked! Your store offers amazing customer service!

Last week I drove to your store to get air in my tires. Team Member Paige discovered the puncture in the tire, and helped me patch the puncture. What service and courtesy. You guys are awesome!

One night, after shopping in your store I found my battery had died. One of your Team Members helped me reach a mechanic and remedy the situation. I will remain a customer for life!

Team Member Marisol carried a customers bags to his waiting taxi. He attempted to tip her, but she politely refused. Acts of generosity and courtesy are rare these days, except when youre at QC!

My son Drew works at QuickChek. When I had to go into the hospital for a few days, Drews team covered his shifts so he could take care of me.

I will only fill my van up at QuickChek because of Team Member Steves courteous and fast service! My kids actually beg to go to his gas pump so they can say hi to Smiley Guy Mr. Steve!

I was looking to get a flu shot but no place took my insurance. Then I called your Pharmacy and was there were two doses left. The Pharmacist even cleared the purchase through my insurance!

After two hours in traffic, I had a flat tire and pulled into your store. Team Member Billy helped try to inflate my tire then recommended and introduced me to a nearby tire repair shop. Such amazingly kindness.

I left my wallet at your store during the morning rush. Later that night, two QC Team Members showed up at my friends house with my wallet! Evidently, the only ID in the wallet was a check from my friend. What caring service!

I purchased some newspapers with a bunch of $1 singles for payment, not realizing a $20 bill was mixed in. Danielle called to me as I was leaving, to return the extra cash. I am so grateful for her integrity!

I purchased a lottery ticket and accidentally overpaid by $10. Jill noticed this error and quickly repaid me. I really appreciate her honesty.

I am an elderly customer who accidently dropped my wallet in your store. Store Leader Dan found my wallet and delivered it to my home! I am so thankful for his honesty and service!

My husband is a transplant recipient. We couldnt get his expensive medication from Walgreens, but your Pharmacist, Justin, got it for us and saved us money with an American-made generic version. Thank you to Justin!

As I pulled away from your fuel pumps, I accidentally ran over a cone. Team Member Michael saw that I couldnt get it unstuck and despite the pouring rain helped me free it! Im so grateful.

My sister unknowingly dropped her phone in your parking lot. A customer found it, and Team Member Anthony called the contacts until one of them told him it was my sisters phone. He got it back to her and we are QC fans forever!

My husband recently ran out of gas a mile away from your store. He was also without his wallet, so your Store Leader gave him money from her wallet to pay for the gas to get him home!

I needed air in my nearly flat tire but couldnt get the cap off of the valve. Your Team helped me, and even put air in all of my tires!

During the blizzard, your team made eight awesome sandwiches for our Public Works crew. Thanks for great service and a job well done!

Administration wanted to provide our staff with warm and nutritious food to keep them going. QuickChek came through with bagels, muffins, mini-loafs and fresh brewed coffee for 75 people!Extremely helpful and delicious!

As I got my morning coffee, I didnt realize I had dropped my money on the ground. Apparently, another customer picked it up and walked out of the store. Your Store Leader paid for my items with no hesitation. I am so grateful.

Team Member Lisa is never too busy to greet me. When I arrived today, I was visibly upset from an argument Id just had. Lisa stopped what she was doing to offer me encouragement. Her kindness meant the world to me.

Team Member put a smile on my disabled daughters face with extra whip cream on her drink! For the experience, I will drive the extra 20 minutes to your store!

Walking into your store always makes me smile. The staff is friendly and the music is great. I dont even smell the cream before I use it- its always fresh. Keep up the great work!

As I fumbled counting out numerous dimes, nickels and pennies to pay for my order, your cashier was so calm and polite. I really appreciated his patience

I was down on my luck and very upset on Tuesday morning. Your Team Member, Joe, was making jokes and made me laugh. He completely changed my attitude.

Id had a really horrible evening, and decided to stop for my favorite pretzel sandwich. There were none but Nia made one right away. I felt welcomed, appreciated, and satisfied.

My husband and I get our coffee and breakfast at your store. This morning we were getting fuel as well. Our bank card declined the full purchase. The cashier, Vanesa, insisted on paying for our coffees herself. It was 2 coffees, but it was a huge, compassionate gesture.

The reason I get gas and coffee at QC is because you offer FREE AIR. Your air stations are well-kept and easy to use. Thank you!

My car ran out of gas a few blocks from your store. Your Team Member, Danny, explained how to put gas in the car with the small can I had purchased. His kindness made my challenging morning a bit sweeter.

QuickChek stores provide one-stop shopping, offering a wide variety of grocery and market items including custom-made, subs, sandwiches, wraps, salads, fresh baked goods and award-winning coffee, served up by excellent locally Hired Team members…READ MORE

Quality Control of Microbiological Culture Media

Quality Control of Microbiological Culture Media

This article first appeared in thePMF Newsletterof January, 2006 and is protected by copyright to PMF. It appears here with permission.

Quality control of microbiological culture media is central to the success of the QC microbiology laboratory (USP 2004). This is reflected by recent changes in the pharmacopeia, both implemented and proposed, have increased the importance of media growth promotion (GP) studies to compendial testing (Cundell 2002, Sutton, 2005). The harmonized Sterility Test (USP 2003a) incorporates requirements for regular sterility testing of media, and the PIC/S recommendation extends this expectation to growth promotion testing of spent media (the Stasis Test see PIC/S 2002). The recently harmonized Microbial Limits Tests (USP 2003b, USP 2003c) expand the requirements to an evaluation of the differential and selective properties of the media, in addition to confirming the nutritive properties. Finally, the proposed USP chapter on microbiological laboratory practices stresses the need to adequately control the growth media (USP 2004). None of these documents, however, provides detailed information on how to establish the overall quality attributes of media.

Although Growth Promotion Testing is the most obvious example of media quality control measures, it is by no means the only measure that a QC microbiology laboratory should employ. In this article we will group the methods used to maintain the quality of microbiological media in four headings:

QC laboratories acquire media in one of two ways, either purchasing the media pre-made from a manufacturer, or making the media (either in whole or in part) in-house. These preparation schemes must be considered separately.

Clearly, if the media is purchased from the vendor there is little opportunity to control the preparation beyond having confidence in the supplier. However, agar acquired in large aliquots for pour-plates must be carefully melted prior to use this melting must be under controlled conditions to avoid damaging the media. Of course, all media used is expected to be checked for physical and chemical parameters and growth promotion (see below), and prepared media is no exception to this expectation.

Media prepared in-house offers several opportunities for quality control. The raw materials (either the dehydrated complete media or the components) must be stored under appropriate and controlled conditions and used within established expiry dates. The compounding of the media must be controlled to ensure the media is prepared correctly. Agar media must be pre-warmed to dissolve the agar prior to sterilization, but not heated so extensively as to damage any heat-labile components. The sterilization procedure also must be under control. Normally this means using a validated autoclave cycle (and load configuration) shown to hold the media at 121oC for 15 minutes (note this is not the same as a 15 minute cycle with a maximum temperature of 121oC). Each batch of media should be clearly labeled to allow for unambiguous audit of each stage of preparation.

The goal of this testing is to provide a gate-keeping function before investing the time in growth-promotion testing. pH of the finished media (pH measurement must be conducted at room temperature unless specific allowance is made for the temperature) is a critical attribute to confirm. The color of the media should be examined and a decision made as to its correctness, as well as an examination for any crystal formations or variations in color (for agars). The containers of media should be thoroughly examined for cracks or defects, and all defective units discarded. There are additional checks that can be performed (HPLC of major components, determination of sugar concentration, etc. Curtis 1985), but these are not normally conducted in the pharmaceutical QC lab.

There are some significant concerns as to the need for GP testing of standard media. It can be argued that since all preparation conditions are under control and the physical parameters of the finished media is checked, there is little additional information gathered by the labor-intensive and time-consuming procedure of checking the growth promoting capabilities of the media. This topic has been debated not only among workers in QC laboratories, but also in the clinical microbiological industry.

Clinical microbiology laboratories in the United States are not required to test most common media under NCCLS standard M22-A2 Quality Assurance for Commercially Prepared Microbiological Culture Media although this stance has come under question by the relevant NCCLS committee and is being re-evaluated (Krishner 1999). The current understanding of which clinical media to test was based on a survey performed in the early 1980s of 1,164 laboratories. From their reported experiences it was determined that most media could be accepted safely on the manufacturers data (Krishner 1999). This result confirmed an earlier study (Nagel and Kunz 1973) that called into question the need for excessive growth-promotion testing of commercially prepared media. They examined 900 lots of 46 different media representing 350,000 units of purchased culture media, and found only 17 lots to be unsatisfactory. These lots were of specialized media containing labile components.

There has been no convincing scientific evidence published that would argue for the need to test Trypticase Soy media, for example, for growth promotion. However, both the Sterility Test and the Microbial Limits Tests require such testing. Given the compendial requirement to test, the first decision may reasonably be to determine the challenge organism. In addition to the compendial organisms required in the tests, addition of specific microorganisms of interest could be useful if they have been recovered from past tests (e.g. a Sterility Test contaminant or a frequent environmental monitoring isolate).

The next concern is test design. There are two types of media commonly used in the microbiological lab broth and agar. These two types must be considered separately as they show growth by completely different means. The fundamental question of GP testing can be expressed as: Is the new batch of media as good as a previously qualified batch? This question cannot be answered adequately except by statistical comparison, given the variability of microbio-logical data. The statistical design of GP studies will be developed in the following discussion which has been influenced by the excellent review by Weenk (1992).

A singular advantage of agar media tests is that they provide numbers colony forming units (CFU). To analyze CFU you must use statistical tools designed for the Poisson distribution (Ilstrup 1990) or else convert the data to approximate the normal distribution. This data conversion can be done by using its log10 values or by taking the square root of (n+1) (Ilstrup 1990). Once this is done, plate counts can be directly compared using Students T Test or other tests of normally distributed data.

There are, of course, several less demanding tests for demonstration of equivalency between two agars:

The compendia assume a GP test by comparison of CFU, with the cells plated in the normal fashion for the lab. The compendia generally require that the colony counts derived from growth on the current batch of media be no less than 50% (USP 2003b) or 70% (USP 2004) of a previously qualified batch. This approach provides the advantages of colony counts and a large area for the colonies to grow, but it is somewhat laborious and expensive in terms of material.

This technique involves dropping the cells in a 10 L aliquot onto the surface of an agar plate (Miles and Misra 1938). When used carefully, an entire 6-fold dilution scheme can be plated in a single Petri dish and if read early, the individual drops can be used to yield estimates of the number of CFU/mL in the challenge suspension. This method offers significant advantages in terms of labor and material resources.

This method is a variation of streaking to extinction. A fresh suspension of the challenge organism is taken into a calibrated loop and streaked in five parallel lines over four sections of an agar plate in sequence, then once through the middle (image from Mossel 1980). These plates are then incubated overnight for growth. The patterns of growth are interpreted to provide an Absolute Growth Index (AGI):

All in quadrants 1, 2, and 3 but half in quadrant 4 and none in middle streak

All in quadrants 1, 2, and 3 but no growth in quadrant 4 or middle streak

Growth scored on half quadrant scores to 2.5, 2.0, 1.5 and so on.

This technique is somewhat operator-dependent and offers a lower precision than those yielding CFU, but can be used to great effect with practice (Mossel 1980).

This is the current compendial method of choice. In this method, the challenge organism is inoculated at a very low level ( 100 CFU per unit) and incubated at the prescribed temperature for the prescribed period of time (3 days or 5 days). Growth in the batch of media is then compared to a parallel sample from a previously qualified batch of the same media. The growth is to be comparable between the two and copious. The advantage of this method is that it does not require a great deal of labor, but the quality of the data for the comparison between the growth promoting characteristics of the media is exceptionally poor. This can be described as a crude end-point test with an n of 1.

In this approach to growth promotion testing, very low levels of inoculum are added to multiple tubes of the two media being examined. Then the resultant growth frequency is compared between the two media to determine equivalency. For example, comparing an old and a new batch of Trypticase Soy Broth (Soy Bean Casein Digest Broth) might be performed by taking 100 tubes of each media, and then inoculating all 200 tubes with 5 CFU of the challenge organism Staphylococcus aureus. After incubation, the number of turbid tubes would be compared say 30/100 of the new media turbid vs. 46/100 of the old media. The statistical comparison could be performed using the Chi Square Test or Fishers Exact Test. This evaluation would be performed separately for each challenge organism. The number of tubes used can be decreased (or increased) at the expense of the statistical power of the method.

End-point methods to growth promotion of broth media are obviously very laborious and technically demanding. It is not difficult to envision a design that would require more than a thousand tubes and the need to accurately create an inoculum of 5 CFU of a variety of challenge microorganisms.

The Most Probable Number method of enumerating microorganisms is most commonly used in the QC lab as part of the Microbial Limits Test (USP 2003b) or in other situations where the sample cannot be put into an appropriate suspension or be filtered (Aspinall and Kilsby 1979). In this technique, the unknown sample is prepared in a ten-fold dilution series and added to nutrient broth in replicate tubes (normally either 3, 5 or 10 replicates are used). The tubes will then either turn turbid (growth) or remain clear, and allow for an estimate of the most probable number of microorganisms. The question being asked in this experimental design is At what point does the unknown number of organisms become so dilute as to fail to inoculate the growth media?

A complete discussion of this technique may be found on the FDA web site as the second appendix to the online version of theBacteriological Analytical Manual( The tables included in this appendix also provide 95% confidence intervals for the estimates of the most probable number.

To use this technique for growth promotion testing you must start with a known concentration of microorganisms and then ask the question Do my two media provide the same estimate of the most probable number of CFU from identical inocula? This is best done by using a low inoculum (approx 50 CFU in the first dilution). The inoculum is serially diluted (ten-fold), and added to the two broths in a 3-tube or a 5-tube design. After incubation, the MPN of the two media are determined (remember, starting from the same inoculum). If the new media is to be qualified, it should not yield an MPN with a confidence interval that is below the lower limit of the confidence interval of the previously qualified batch.

This technique for growth promotion testing of broths offers the advantages of being much less expensive in terms of time and resources than the other broth techniques (with the exception of the compendial tests) as well as being very forgiving about the concentration of the starting inoculum. It is by far the easiest method to provide a statistical comparison between the growth promoting capabilities of two broth media batches. This approach can be made much easier as well by using commercially available starting inocula of defined numbers (such as BTFs BioBall Morgan 2004).

The growth promoting capabilities of two batches of broth can be compared by measuring the growth curves of identical inocula grown side-by-side. The growth rate of the challenge organism in the broth can be determined either spectrophotometrically or by viable count to provide a sensitive means to compare the nutritive properties of the media. However, this method is extremely labor intensive. A second method using kinetic parameters is to compare the length of the lag phases of the same inoculum on the two media. The comparison of lag phase measurements suffers the same disadvantage of labor usage, and can be very difficult to implement and subject to significant variability. Neither of these methods is practical for the QC microbiology lab due to their high labor requirements.

The best overall design for GP studies of agar media would be through the Miles-Misra technique as it is economical (both in material and labor), and provides colony counts. The best design for GP studies of broth media is the MPN design which allows statistical comparison between the media batches without requiring large investments of time and material.

The laboratory must have some procedures in place to prevent unqualified media from entering the testing process. This ideally would be a separate storage room from that used to store qualified media, but may also be accomplished through tagging the quarantined material and placing it in a clearly identified area within the same room. All quality control checks on the quarantined media should be completed before its documented release for general use. Storage conditions of the quarantined media should match those of the released media.

Media should always be stored under controlled conditions to ensure its quality through to the expiry date. Factors to be evaluated in these controlled conditions include:

Although all these factors may not be a concern for all media, they can be a concern for different types. For example, Trypticase Soy Agar is robust and can tolerate a wide range of storage conditions (assuming appropriate temperature control) though the stability period. However, Dey-Engley Broth (Dey Engley 1983), a broth commonly used to neutralize a variety of disinfectants and preservatives, will degrade upon exposure to oxygen and so must be stored in oxygen-impermeable material in well-sealed container. Similarly, Fluid Thioglycollate Medium needs to maintain a highly reduced state for recovery of anaerobes and so must have oxygen excluded. This medium, used in the Sterility Test (USP 2003a) incorporates resazurin as a redox indicator which turns the medium pink if exposed to oxygen. The Sterility Test procedure calls for action if the upper third of the media is pink in color.

The expiry date of media may be set by the vendor of purchased media, but must be established by the lab for in-house media. This dating can draw upon the compendia for guidance. The Sterility Test states that:

If prepared media are stored in unsealed containers, they can be used for 1 month, provided that they are tested for growth promotion within 2 weeks of the time of use and that color indicator requirements are met. If stored in tight containers, the media can be used for 1 year, provided that they are tested for growth promotion within 3 months of the time of use and that the color indicator requirements are met.

Finally, the proposed informational chapter 1117 Best Microbiological Laboratory Practices (USP 2004) devotes an entire section to media storage and can also be used to develop a defendable expiry dating policy.

We have examined four points to a quality control program for microbiological culture media:

The importance of maintaining the quality of the media cannot be overstated there are few things in the QC microbiology laboratory that will lead to problems with every aspects of the operation, and media is at or near the top of that very short list. Time spent ensuring culture media performance will be amply repaid in terms of data reproducibility and minimizing time spent on investigations of non-conforming results.

Aspinall, L.J. and DC Kilsby. 1979. A Microbiological Quality Control Procedure Based On Tube Counts. J Appl Bacteriol. 46:325-329.

Cundell, A. 2002. Review of the Media Selection and Incubation Conditions for the Compendial Sterility and Microbial Limit Tests. Pharm Forum Nov/Dec 2002. 28(6):2034- 2041

Curtis, G.D.W. 1985. A Review of Methods for Quality Control of Culture Media. Int J Food Microbiol. 2:13-20.

Dey, B.P.; F.B. Engley. 1983. Methodology for Recovery of Chemically Treated Staphylococcus aureus with Neutralizing Medium. Appl Environ Microbiol. 45(5):1533-1537.

Eisenhart, C and W Perry. 1943. Statistical Methods and Control In Bacteriology. Bacteriol Rev. 7:57-137.

Ilstrup, D. 1990. Statistical Methods In Microbiology. Clin Microbiol Rev. 3(3):219-226.

Krisher, K. 1999. The Quality Control of Microbiological Media Revisited: Is It Time for a Change? Clin Microbiol Newsl. 21(20):161-162

Miles, A.A. and SS Misra. 1938. Estimation of the Bactericidal Power of the Blood. J Hyg (Camb) 38:732-749.

Morgan, CA et al. 2004. Production of Precise Microbiology Standards Using Flow Cytometry and Freeze Drying. Cytometry. Part A 62A:162-168

Mossel, D.A.A. 1980. Quality Control of Solid Culture Media: a Comparison of the Classic and the So-Called Ecometric Technique. J Appl Bacteriol. 49:439-454.

Nagel, J. G. and L. J. Kunz. 1973. Needless Retesting of Quality-Assured, Commercially Prepared Culture Media. Appl Microbiol. 26(1):31-37.

PIC/S. 2002. Recommendation On Sterility Testing.

Sutton, SVW, et al. 2005. Activities of the USP Analytical Microbiology Expert Committee During the 2000-2005 Revision Cycle. PDA J Pharm Sci Tech. 59(3):157-176.

USP. 2003a. 71 Sterility Test Interim Announcement. Pharm Forum. Jul/Aug 2003 29(4):933-940.

USP. 2003b. 61 Microbiological Examination Of Nonsterile Products: Microbial Enumeration Tests. Pharm Forum. Sept/Oct 2003. 29(5):1714-1722.

USP. 2003c. 62 Microbiological Examination of Nonsterile Products: Tests for Specified Microorganisms Harmonization. Pharm Forum. Sept/Oct 2003. 29(5): 1722-1735.

USP. 2004. 1117 Microbiological Best Laboratory Practices. Pharm Forum. Sept/Oct 2004. 30(5):1713-1721.

Weenk, G.H. 1992. Microbiological Assessment of Culture Media: Comparison and Statistical Evaluation of Methods. Int J Food Microbiol. 17:159-181.

Experts at the Microbiology Network are ready to assist with consulting or training to meet your needs.  Have customized, in-house assistance with your questions from our recognized experts at your schedule either through consulting agreements, in-house training or customized webinars contact us using the Lets Talk communication found in the upper right of this page.